NY-ESO-1 tumour associated antigen is a cytoplasmic protein detectable by specific monoclonal antibodies in cell lines and clinical specimens
2000
Detecting NY-ESO-1 Protein in Melanoma
Sample size: 12
publication
Evidence: moderate
Author Information
Author(s): Schultz-Thater E, Noppen C, Gudat F, Dürmüller U, Zajac P, Kocher T, Heberer M, Spagnoli G C
Primary Institution: Research Division, Department of Surgery, University of Basel
Hypothesis
Can specific monoclonal antibodies detect the NY-ESO-1 protein in melanoma specimens?
Conclusion
The study found that NY-ESO-1 is a cytoplasmic protein in melanoma cells, suggesting its potential as a target for immunotherapy.
Supporting Evidence
- NY-ESO-1 is identified as a 22 kDa protein in melanoma cell lines.
- Specific staining was limited to neoplastic cells in melanoma specimens.
- In five cases with NY-ESO-1 expression, over 90% of tumor cells showed positive staining.
Takeaway
Researchers found a protein in melanoma that could help doctors create better treatments for patients.
Methodology
Generated specific monoclonal antibodies to identify NY-ESO-1 in melanoma cell lines and clinical specimens.
Digital Object Identifier (DOI)
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