How Fish Oil Affects Inflammation in Stem Cells from Human Fat
Author Information
Author(s): Simao Jussara de Jesus, Bispo Andressa França de Sousa, Plata Victor Tadeu Gonçalves, Abel Ana Beatriz Marques, Saran Raphael Justa, Barcella Júlia Fernandes, Alonso João Carlos Cardoso, Santana André Valente, Alonso-Vale Maria Isabel Cardoso
Primary Institution: Federal University of São Paulo—UNIFESP
Hypothesis
In a low-grade inflammatory environment, typical of obesity, NF-κB modulates the expression of histone modifiers in adipose-derived stem cells.
Conclusion
Fish oil demonstrates potential in modulating inflammation-induced epigenetic changes and preserving adipocyte function.
Supporting Evidence
- LPS increased NF-κB activity, elevating KDM6B demethylase levels and H3K27 acetylation.
- Fish oil mitigated the effects of LPS, reducing H3K27 acetylation and promoting methylation.
- Chronic exposure to LPS established an epigenetic memory in adipose-derived stem cells.
Takeaway
This study shows that fish oil can help protect fat stem cells from inflammation caused by obesity, which can help them work better.
Methodology
Human visceral adipose-derived stem cells were stimulated with lipopolysaccharide and treated with fish oil, followed by various assays including flow cytometry and PCR.
Potential Biases
Potential bias due to the specific population sample and the controlled laboratory conditions.
Limitations
The study had a small sample size and results may not be generalizable to all populations.
Participant Demographics
Four male patients aged between 30 and 50 years, all overweight.
Statistical Information
P-Value
0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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