Discovery of a Nitrile Hydratase in Monosiga brevicollis
Author Information
Author(s): Konrad U. Foerstner, Tobias Doerks, Jean Muller, Jeroen Raes, Peer Bork
Primary Institution: European Molecular Biology Laboratory
Hypothesis
Is there a nitrile hydratase present in the eukaryote Monosiga brevicollis?
Conclusion
The study identifies the first nitrile hydratase in a eukaryote, suggesting ancient horizontal gene transfer from bacteria.
Supporting Evidence
- The NHase was found in Monosiga brevicollis, a unicellular model organism.
- Phylogenetic analyses suggest ancient horizontal gene transfer from proteobacteria.
- The presence of an intron and expressed sequence tags support the functionality of the gene.
Takeaway
Scientists found a special enzyme in a tiny organism called Monosiga brevicollis that helps break down certain chemicals, showing that this enzyme can exist in living things that are not bacteria.
Methodology
The study involved a large-scale computational screening of sequence data to identify nitrile hydratases.
Limitations
The exact natural substrate of Monosiga's NHase could not be predicted, and low concentrations of nitriles in its habitats may hinder further study.
Digital Object Identifier (DOI)
Want to read the original?
Access the complete publication on the publisher's website