Presence of Mitochondrial Inhibitory Factor 1 in Human Serum and Its Correlation with HDL-Cholesterol
Author Information
Author(s): Annelise Genoux, Véronique Pons, Claudia Radojkovic, Florence Roux-Dalvai, Guillaume Combes, Corinne Rolland, Nicole Malet, Bernard Monsarrat, Frédéric Lopez, Jean-Bernard Ruidavets, Bertrand Perret, Laurent O. Martinez
Primary Institution: INSERM, UMR1048, Institut de Maladies Métaboliques et Cardiovasculaires, Toulouse, France
Hypothesis
Is Mitochondrial Inhibitory Factor 1 (IF1) present in human serum and does it correlate with serum lipid levels?
Conclusion
The study found that circulating IF1 levels are positively correlated with HDL-cholesterol and negatively correlated with triglycerides.
Supporting Evidence
- IF1 was detected in human serum using immunoprecipitation and mass spectrometry.
- Serum IF1 levels showed a normal distribution with a median value of 0.49 µg/mL.
- Higher IF1 levels were associated with increased HDL-cholesterol and decreased triglycerides.
Takeaway
This study shows that a protein called IF1 is found in human blood and it seems to help keep good cholesterol levels high while lowering bad fat levels.
Methodology
The presence of IF1 in serum was detected using immunoprecipitation and mass spectrometry, and its levels were quantified using a competitive ELISA assay.
Limitations
The study was limited to a specific population of healthy normolipemic male subjects, which may not be representative of the general population.
Participant Demographics
100 healthy normolipemic male subjects, average age 59.1 years.
Statistical Information
P-Value
0.009
Confidence Interval
0.22–0.82 µg/mL
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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