Feeding Cues and Nutrients Affect Clock Genes in Mouse Liver
Author Information
Author(s): Oike Hideaki, Nagai Kanji, Fukushima Tatsunobu, Ishida Norio, Kobori Masuko
Primary Institution: National Food Research Institute, National Agriculture and Food Research Organization, Tsukuba, Japan
Hypothesis
The study investigates how feeding cues and injected nutrients influence the expression of clock genes in the liver and lungs of mice.
Conclusion
The study found that multiple clock genes in the liver respond rapidly to nutritional cues within one hour, while the lung clock remains unaffected.
Supporting Evidence
- The liver clock can adapt rapidly to new feeding cycles within a few days.
- A single delayed feeding significantly affected the phase of the liver clock.
- Clock genes like Per2 and Dec1 were significantly increased within 1 hour of feeding.
Takeaway
When mice eat or get nutrients, their liver clock genes wake up quickly, but the lung doesn't react the same way.
Methodology
The study used Per2::Luc knock-in mice to examine the effects of delayed feeding and nutrient injections on clock gene expression in the liver and lungs.
Limitations
The study primarily focused on male mice and may not generalize to females or other species.
Participant Demographics
Male BALB/cAn mice, aged 10–30 weeks.
Statistical Information
P-Value
p<0.001
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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