Virtual histology of transgenic mouse embryos for high-throughput phenotyping
2006

3-D Imaging of Mouse Embryos

Sample size: 120 publication Evidence: high

Author Information

Author(s): Johnson JT, Hansen MS, Wu I, Healy LJ, Johnson CR, Jones GM

Primary Institution: Children’s Cancer Research Institute, University of Texas Health Sciences Center at San Antonio

Hypothesis

Can a new method improve the assessment of birth defects in mouse embryos?

Conclusion

The new microCT-based virtual histology technique allows for rapid, high-resolution imaging of mouse embryos, improving the study of genetic mutations and chemical effects.

Supporting Evidence

  • The new technique allows for the examination of internal organs and structures that traditional methods cannot visualize.
  • MBVH can analyze up to 120 embryos simultaneously, significantly speeding up the research process.
  • The cost per scan with MBVH is $1.50, compared to $600 for traditional methods.

Takeaway

Scientists can now take 3-D pictures of mouse embryos quickly and easily, helping them understand how genes affect development.

Methodology

The study used microCT scans combined with computer visualization techniques to create detailed 3-D images of mouse embryos.

Participant Demographics

Mouse embryos with genetic mutations.

Digital Object Identifier (DOI)

10.1371/journal.pgen.0020061

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