Differential Development of Neuronal Responsiveness in Human Neural Stem Cells
Author Information
Author(s): Donato Roberta, Miljan Erik A, Hines Susan J, Aouabdi Sihem, Pollock Kenneth, Patel Sara, Edwards Frances A, Sinden John D
Primary Institution: University College London
Hypothesis
Using immortalized stem cell lines, cells prevented from differentiation by the presence of growth factors would lack neuronal phenotype.
Conclusion
Overexpression of myc in neural stem cells can generate electrophysiologically active neurons in culture, but the ability to develop functional neurons varies between different cell lines.
Supporting Evidence
- Both ReNcell VM and CX are nestin positive and have resting membrane potentials of around -60 mV.
- ReNcell VM can form neurons, astrocytes, and oligodendrocytes, while ReNcell CX does not develop the same electrophysiological properties.
- 100% of ReNcell VM featured TTX-sensitive Na+-channels and fired action potentials after a week of differentiation with the preD protocol.
Takeaway
Scientists are trying to grow brain cells in a lab that can work like real neurons. They found that some cells can become active and send signals, while others can't.
Methodology
The study used two human neural stem cell lines, ReNcell VM and CX, and tested their differentiation potential and functional capacity under different culture conditions.
Limitations
The study does not explore the specific factors that may affect the differentiation potential of the cell lines.
Digital Object Identifier (DOI)
Want to read the original?
Access the complete publication on the publisher's website