Monoclonal Antibody SZ34 Reduces von Willebrand Factor Proteolysis
Author Information
Author(s): Zhang Jingyu, Ma Zhenni, Dong Ningzheng, Liu Fang, Su Jian, Zhao Yiming, Shen Fei, Wang Anyou, Ruan Changgeng
Primary Institution: Jiangsu Institute of Hematology, The First Affiliated Hospital of Soochow University, Suzhou, China
Hypothesis
Do monoclonal antibodies against von Willebrand factor affect its susceptibility to proteolysis by ADAMTS13?
Conclusion
The monoclonal antibody SZ34 inhibits the proteolytic cleavage of von Willebrand factor by ADAMTS13 under shear stress conditions.
Supporting Evidence
- SZ34 inhibited proteolytic cleavage of VWF by ADAMTS13 in a concentration-dependent manner under shear stress.
- The binding epitope of SZ34 mAb is located between A1555 and G1595 in the central A2 domain of VWF.
- Other anti-VWF mAbs had no effects on the proteolytic cleavage of VWF by ADAMTS13.
Takeaway
A special antibody can help protect a blood protein from being broken down too quickly when blood flows fast.
Methodology
The study used eight monoclonal antibodies to evaluate their effects on von Willebrand factor cleavage by ADAMTS13 under different conditions.
Limitations
The effects of SZ34 were not observed under static or denaturing conditions.
Digital Object Identifier (DOI)
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