Reprogrammed Transcriptome in Rhesus-Bovine Interspecies Somatic Cell Nuclear Transfer Embryos
Author Information
Author(s): Wang Kai, Otu Hasan H., Chen Ying, Lee Young, Latham Keith, Cibelli Jose B.
Primary Institution: Michigan State University
Hypothesis
Patterns of gene expression failures in iSCNT will emerge, potentially indicating ways to improve the reprogramming procedures in somatic cells.
Conclusion
iSCNT embryos are capable of triggering embryonic genome activation, but some somatic genes remain expressed, indicating incomplete nuclear reprogramming.
Supporting Evidence
- Two thousand and seven genes were activated in iSCNT embryos.
- One quarter of these genes reached expression levels comparable to IVF embryos.
- Eight hundred and sixty somatic genes were not silenced properly in iSCNT embryos.
Takeaway
Scientists are studying how to make embryos from different species work better together, and they found that some genes don't turn off like they should.
Methodology
The study analyzed the EGA-related transcriptome of rhesus-bovine iSCNT embryos using gene array analysis.
Potential Biases
Potential bias in gene expression analysis due to the use of different species' cells.
Limitations
The study did not assess the long-term viability of the embryos beyond the 8- to 16-cell stage.
Participant Demographics
Rhesus monkey fibroblasts and bovine oocytes were used in the study.
Statistical Information
P-Value
2.76E-10
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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