Identification of genes differentially expressed in association with acquired cisplatin resistance
2000
Identifying Genes Linked to Cisplatin Resistance
publication
Author Information
Author(s): Johnsson A, Zeelenberg I, Min Y, Hilinski J, Berry C, Howell S B, Los G
Primary Institution: University of California, San Diego
Hypothesis
The study aims to identify genes whose mRNA levels are differentially expressed in human cells with acquired cisplatin resistance.
Conclusion
The study identified several genes associated with cisplatin resistance that had not been previously linked to this phenotype.
Supporting Evidence
- The study identified 51 cDNA fragments in the UP library and 16 in the DOWN library that were differentially expressed.
- 87% of the identified cDNA fragments were sequenced and found in Genbank.
- High levels of differential expression were observed for several key genes linked to cisplatin resistance.
Takeaway
Researchers looked for genes that change when cancer cells become resistant to a drug called cisplatin, and they found many new ones.
Methodology
The study used suppressive subtraction hybridization (SSH) to create cDNA libraries from resistant and non-resistant cell lines.
Digital Object Identifier (DOI)
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