Comparing Stem Cells from Transgenic and Non-Transgenic Mice
Author Information
Author(s): Ripoll Cynthia B, Bunnell Bruce A
Primary Institution: Tulane University Health Sciences Center
Hypothesis
How do mesenchymal stem cells from eGFP transgenic mice compare to those from non-transgenic mice in terms of differentiation and characterization?
Conclusion
Murine adipose derived and bone marrow derived mesenchymal stem cells from non-transgenic and eGFP transgenic C57Bl/6 mice have very similar characterization profiles.
Supporting Evidence
- The expression of eGFP does not appear to affect the ability to differentiate along adipogenic or osteogenic lineages.
- mMSCs derived from both bone marrow and adipose tissue had similar surface marker profiles.
- There were no significant differences between transgenic and non-transgenic mMSCs.
Takeaway
This study looked at two types of stem cells from mice and found that they are very similar, even when one type has a special green marker that helps scientists see them better.
Methodology
The study compared the differentiation potential, colony forming unit capabilities, cell surface marker profiles, and growth kinetics of mesenchymal stem cells from both transgenic and non-transgenic mice.
Limitations
The study was limited by the small sample size of donors, which may affect the generalizability of the results.
Participant Demographics
Cells were obtained from 3–4 donors of both sexes, aged 2–4 months.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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