Radiation-Induced Cisplatin Resistance in Mouse Fibrosarcoma Cells
Author Information
Author(s): H. Eichholtz-Wirth, G. Reidel, B. Hietel
Primary Institution: Institut für Strahlenbiologie, GSF
Hypothesis
Can cisplatin resistance be induced in murine fibrosarcoma cells through radiation exposure?
Conclusion
Cisplatin resistance in SSK cells can be induced by radiation and is associated with increased metallothionein content.
Supporting Evidence
- Transient cisplatin resistance can be induced by radiation exposure.
- Both radiation-induced and drug-induced resistant cells showed increased metallothionein content.
- Resistance was not associated with a decrease in radiosensitivity.
- Loss of cisplatin resistance occurred after 10 to 25 generations.
- SSK-rad cells exhibited enhanced drug resistance compared to parental SSK cells.
Takeaway
Scientists found that some cancer cells can become resistant to a drug called cisplatin after being exposed to radiation, and this resistance is linked to a substance called metallothionein.
Methodology
Mouse fibrosarcoma cells were exposed to fractionated gamma irradiation and then tested for drug and radiation sensitivity.
Limitations
The study did not systematically determine the induction rate of drug resistance after irradiation.
Participant Demographics
Murine fibrosarcoma cells were used in the study.
Statistical Information
P-Value
p<0.01
Statistical Significance
p<0.05
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