Study of Odorant Receptor Genes in Large Yellow Croaker
Author Information
Author(s): Zhou Yingsong, Yan Xiaojun, Xu Shanliang, Zhu Peng, He Xianxing, Liu Jianxin
Primary Institution: Zhejiang University
Hypothesis
It is necessary to clone the OR mRNAs directly from the olfactory epithelium and to examine their expression status.
Conclusion
About 100 OR genes are expressed in the olfactory epithelial tissues of L. crocea, indicating their evolutionary relationships and varying roles in olfactory function.
Supporting Evidence
- Eighty-nine full-length and 22 partial OR cDNA sequences were isolated from the olfactory epithelium.
- The OR genes of L. crocea are closely related to those of fugu, pufferfish, and stickleback.
- The different expression levels of OR genes suggest varying roles in olfactory function.
Takeaway
The large yellow croaker has about 100 genes that help it smell things, and these genes are important for how it interacts with its environment.
Methodology
The study involved cloning OR cDNAs from the olfactory epithelium and conducting phylogenetic analysis and quantitative real-time PCR.
Limitations
The study does not confirm the transcriptional activity of all identified OR genes, and some may not be expressed.
Participant Demographics
One-year-old large yellow croakers were collected from Xiangshan Bay in Zhejiang province, China.
Statistical Information
P-Value
p<0.001
Statistical Significance
p<0.001
Digital Object Identifier (DOI)
Want to read the original?
Access the complete publication on the publisher's website