Myo-inositol Improves Development in Pig Embryos
Author Information
Author(s): Jawad Ali, Oh Dongjin, Choi Hyerin, Kim Mirae, Ham Jaehyung, Oh Byoung Chol, Lee Joohyeong, Hyun Sang-Hwan
Primary Institution: Chungbuk National University
Hypothesis
Myo-inositol enhances the developmental competence of porcine parthenogenetic embryos and reduces oxidative stress.
Conclusion
Myo-inositol supplementation significantly improves blastocyst development and mitochondrial function in porcine embryos.
Supporting Evidence
- Myo-inositol at 10 and 20 mM significantly increased the blastocyst formation rate compared to the control group.
- Embryos with 20 mM Myo-inositol had higher glutathione levels and lower reactive oxygen species levels.
- Myo-inositol reduced the rate of apoptosis in blastocysts.
- 20 mM Myo-inositol improved mitochondrial membrane potential and quantity.
- Gene expression related to mitochondrial function and the NRF2 pathway was elevated in Myo-inositol treated groups.
Takeaway
Myo-inositol helps pig embryos grow better and stay healthy by reducing stress and improving their energy factories, called mitochondria.
Methodology
Various concentrations of Myo-inositol were added to the porcine zygotic medium during in vitro culture of embryos, and several developmental characteristics were evaluated.
Limitations
The study primarily focused on specific concentrations of Myo-inositol and may not account for other potential factors affecting embryo development.
Participant Demographics
Porcine embryos derived from parthenogenetic activation.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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