Formulation of a killed whole cell pneumococcus vaccine - effect of aluminum adjuvants on the antibody and IL-17 response
2011

Pneumococcal Vaccine with Aluminum Adjuvants

Sample size: 8 publication Evidence: moderate

Author Information

Author(s): HogenEsch Harm, Dunham Anisa, Hansen Bethany, Anderson Kathleen, Maisonneuve Jean-Francois, Hem Stanley L

Primary Institution: Purdue University

Hypothesis

The study aims to determine the optimal formulation of a killed whole cell pneumococcal vaccine with aluminum-containing adjuvants for intramuscular injection.

Conclusion

The formulation of the whole killed bacterial cells with aluminum hydroxide resulted in a stable vaccine that induced both antibodies and an IL-17 response.

Supporting Evidence

  • All adjuvants enhanced the antibody response over WCA alone.
  • The highest concentration of anti-WCA IgG was observed in mice injected with WCA/AP.
  • Vaccines formulated with AH induced an IL-17 response.
  • The vaccine formulated with AH was stable and retained full immunogenicity when stored at 4°C for 4 months.

Takeaway

Researchers created a new vaccine to protect against a common bacteria that can make people very sick, and found that using a special ingredient helped the body fight it better.

Methodology

Mice were immunized with different formulations of a killed whole cell pneumococcal vaccine combined with various aluminum adjuvants, and their antibody and IL-17 responses were measured.

Limitations

The study primarily involved mice, which may not fully represent human responses.

Participant Demographics

Seven week old female C57BL/6J mice were used in the experiments.

Statistical Information

P-Value

p<0.05

Statistical Significance

p<0.05

Digital Object Identifier (DOI)

10.1186/1476-8518-9-5

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