Choosing Reference Genes for Studying Atlantic Cod
Author Information
Author(s): Pål A. Olsvik, Liv Søfteland, Kai K. Lie
Primary Institution: National Institute of Nutrition and Seafood Research
Hypothesis
Reference genes are stable in tissues within and between populations of cod living in contaminated habitats.
Conclusion
The study suggests that Ubi and ARP can be useful as reference genes in qRT-PCR examination of gene expression in wild populations of Atlantic cod.
Supporting Evidence
- The study evaluated 10 potential reference genes in six tissues of Atlantic cod.
- Ubi and ARP were consistently ranked as the most stable reference genes across different tissues.
- GAPDH and ACTB were ranked as the least stable reference genes in all examined tissues.
Takeaway
Scientists looked at different genes in Atlantic cod to find the best ones to use for measuring other genes. They found that two genes, Ubi and ARP, are really good for this.
Methodology
The stability of 10 potential reference genes was examined in six tissues of Atlantic cod from four populations using qRT-PCR and analyzed with geNorm and NormFinder tools.
Limitations
The study only examined six juvenile fish from three wild populations and farmed Atlantic cod, which may limit the generalizability of the findings.
Participant Demographics
Six juvenile Atlantic cod from four populations, including two from contaminated areas and one from an aquaculture facility.
Digital Object Identifier (DOI)
Want to read the original?
Access the complete publication on the publisher's website