Genetic Monitoring in Laboratory Mice Using Luminex xTAG Assay
Author Information
Author(s): Zhou Jiaqi, Wei Jie, Wang Hong, Li Huan, Zhao Lan, Fu Rui, Yue Bingfei, Pavlovic Guillaume
Primary Institution: National Institutes for Food and Drug Control, Beijing, China
Hypothesis
The study aims to develop novel panels of SNP markers for genetic monitoring in inbred and outbred laboratory mice.
Conclusion
The developed panels provide a reliable method for genetic monitoring of laboratory mice, ensuring research quality.
Supporting Evidence
- The study tested commonly used inbred strains and five DNA samples from the International Council for Laboratory Animal Science.
- Results showed that the Luminex xTAG Assay is a reliable tool for accurately identifying targeted SNPs.
- Genotyping results indicated that all strains were homozygous at all 34 loci, with no heterozygotes present.
Takeaway
This study created a new way to check the genes of lab mice, which helps scientists make sure their experiments are accurate.
Methodology
The study used Luminex xTAG assay to test SNP loci in inbred and outbred mice, comparing results with traditional STR markers.
Limitations
The number of SNP loci selected is small, and further studies should expand the number of SNPs for outbred stocks.
Participant Demographics
Inbred strains were selected from various sources in Beijing and Shanghai, and outbred groups were obtained from the National Rodent Laboratory Animal Resources Center.
Digital Object Identifier (DOI)
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