The Role of RamA in Ciprofloxacin Resistance in Salmonella
Author Information
Author(s): Sun Yawei, Dai Menghong, Wang Haihong, Huang Yulian, Almofti Lingli, Liu Yassir A., Yuan Zhenli, Zonghui
Primary Institution: MOA Key Laboratory of Food Safety Evaluation/National Reference Laboratory of Veterinary Drug Residues (HZAU), Huazhong Agricultural University, Wuhan, Hubei, People's Republic of China
Hypothesis
The study aims to elucidate the roles of RamA on the development of ciprofloxacin resistance in Salmonella enterica serovar Typhimurium.
Conclusion
The expression of RamA promotes the development of ciprofloxacin resistant mutants of Salmonella enterica serovar Typhimurium.
Supporting Evidence
- RamA is an essential element in producing multidrug resistant Salmonella enterica serovar Typhimurium.
- Four spontaneous mutants were obtained during the study.
- RamA was shown to control susceptibility to ciprofloxacin by activating efflux pumps.
- Inhibition of RamA could decrease the appearance of ciprofloxacin resistant mutants.
Takeaway
This study found that a protein called RamA helps Salmonella bacteria become resistant to a medicine called ciprofloxacin, which is used to treat infections.
Methodology
Spontaneous mutants were selected from a susceptible strain of Salmonella under increasing concentrations of ciprofloxacin, and various tests were conducted to assess resistance mechanisms.
Digital Object Identifier (DOI)
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