Study of Genes Affected by H5N1 Avian Influenza in Different Hosts
Author Information
Author(s): Balasubramaniam Vinod RMT, Hassan Sharifah S, Omar Abdul R, Mohamed Maizan, Noor Suriani M, Mohamed Ramlan, Othman Iekhsan
Primary Institution: Monash University
Hypothesis
How does the H5N1 Avian Influenza virus affect gene expression in different host systems?
Conclusion
The study identified 37 differentially expressed genes in response to H5N1 infection across three host systems, indicating varied responses.
Supporting Evidence
- The study identified 37 known and unique differentially expressed genes.
- The Gene Fishing™ technique allowed for the isolation and identification of host cellular genes regulated during H5N1 infection.
- Differentially expressed genes were not identical across the three host systems, suggesting different mechanisms of response.
Takeaway
The study looked at how a virus called H5N1 changes the genes in chickens and other cells, finding that different cells react in different ways.
Methodology
The study used a differential display technique called Gene Fishing™ to identify differentially expressed genes in infected cells and tissues.
Limitations
The study was limited by the small number of differentially expressed genes that could be captured on agarose gels.
Participant Demographics
Six specific pathogen free chickens were used in the study.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
Want to read the original?
Access the complete publication on the publisher's website