Identification and characterization of Smyd2: a split SET/MYND domain-containing histone H3 lysine 36-specific methyltransferase that interacts with the Sin3 histone deacetylase complex
2006
Understanding Smyd2: A New Protein That Affects Gene Regulation
publication
Evidence: moderate
Author Information
Author(s): Mark A Brown, Robert J Sims III, Paul D Gottlieb, Philip W Tucker
Primary Institution: University of Texas at Austin
Hypothesis
Smyd2 is a histone methyltransferase that interacts with the Sin3 histone deacetylase complex and regulates gene expression.
Conclusion
Smyd2 dimethylates histone H3 at lysine 36 and is linked to the repression of cell proliferation.
Supporting Evidence
- Smyd2 is most highly expressed in heart and brain tissue.
- Smyd2 represses transcription from an SV40-luciferase reporter.
- Over-expression of Smyd2 suppresses cell proliferation.
Takeaway
Smyd2 is a protein that helps control how genes are turned on and off, and it can slow down how fast cells grow.
Methodology
The study involved northern analysis, in situ hybridization, immunohistochemical staining, and histone methyltransferase assays.
Digital Object Identifier (DOI)
Want to read the original?
Access the complete publication on the publisher's website