How Complement Opsonization Affects HIV-1 Uptake by Dendritic Cells
Author Information
Author(s): Tjomsland Veronica, Ellegård Rada, Che Karlhans, Hinkula Jorma, Lifson Jeffrey D., Larsson Marie
Primary Institution: Linköping University, Linköping, Sweden
Hypothesis
Complement opsonization enhances the uptake of HIV-1 by dendritic cells.
Conclusion
Complement opsonization significantly increases the internalization of HIV-1 by dendritic cells compared to free virus.
Supporting Evidence
- C-HIV significantly enhanced the uptake by immature and mature dendritic cells.
- The internalization rate was dependent on both opsonization of the virus and dendritic cell maturation state.
- Blocking integrins significantly reduced the binding and uptake of both free and complement opsonized HIV-1.
Takeaway
When HIV-1 is covered in special proteins from the immune system, it gets taken up by certain immune cells much better than when it's not covered.
Methodology
The study compared the binding and internalization of free HIV-1 and complement opsonized HIV-1 by immature and mature dendritic cells using p24 ELISA.
Limitations
The study primarily focused on in vitro conditions, which may not fully replicate in vivo scenarios.
Statistical Information
P-Value
p<0.005
Statistical Significance
p<0.005
Digital Object Identifier (DOI)
Want to read the original?
Access the complete publication on the publisher's website