Collagen V siRNA Engineered Tenocytes for Tendon Tissue Engineering
Author Information
Author(s): Lu Ping, Zhang Guo Rong, Song Xing Hui, Zou Xiao Hui, Wang Lin Lin, Ouyang Hong Wei
Primary Institution: Zhejiang University, Hangzhou, China
Hypothesis
The study investigates the effect of specific chains of collagen V on the fibrillogenesis of tenocytes and the efficacy of Col V siRNA engineered tenocytes for tendon tissue engineering.
Conclusion
Col V siRNA engineered tenocytes improved tendon tissue regeneration and an optimal level of collagen V is vital in regulating collagen fibrillogenesis.
Supporting Evidence
- Down regulation of collagen V α1 or α2 chains by siRNAs had different effects on collagen I and decorin gene expressions.
- Col5α1 siRNA treated tenocytes had smaller collagen fibrils with abnormal morphology.
- Tendons formed by coculture of Col5α1 siRNA treated tenocytes with normal tenocytes at a proper ratio had larger collagen fibrils.
Takeaway
This study shows that using special treatments on tendon cells can help them heal better by making the right kind of collagen.
Methodology
The study used RNA interference gene therapy and a scaffold-free tissue engineered tendon model to assess the effects of collagen V chains on tenocyte behavior.
Limitations
The precise optimal level of collagen type V for tendon repair remains undetermined and warrants further investigation.
Participant Demographics
Sprague-Dawley rats were used for isolating tenocytes.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
Want to read the original?
Access the complete publication on the publisher's website