LcrG secretion is not required for blocking of Yops secretion in Yersinia pestis
2008
LcrG Secretion and Its Role in Yersinia pestis
publication
Evidence: moderate
Author Information
Author(s): Reina Laura D, O'Bryant Deanna M, Matson Jyl S, Nilles Matthew L
Primary Institution: University of North Dakota, School of Medicine and Health Sciences
Hypothesis
Is LcrG secretion necessary for its function in Yersinia pestis?
Conclusion
LcrG secretion is not required for its ability to control secretion in Yersinia pestis.
Supporting Evidence
- LcrG was shown to be secreted into the growth medium in the absence of calcium.
- Mutant LcrG proteins were functional in regulating Yops secretion despite not being secreted.
- The study confirmed that LcrG secretion is mediated by the Yersinia T3SS.
Takeaway
The study found that LcrG can work even if it doesn't get secreted, which means its secretion isn't important for its job.
Methodology
The study involved constructing LcrG chimeras and mutants to analyze their secretion and function in Yersinia pestis.
Limitations
The variability in LcrG secretion across different Yersinia species complicates the conclusions.
Digital Object Identifier (DOI)
Want to read the original?
Access the complete publication on the publisher's website