ES-Cell Derived Hematopoietic Cells Induce Transplantation Tolerance
Author Information
Author(s): Bonde Sabrina, Chan Kun-Ming, Zavazava Nicholas
Primary Institution: University of Iowa Roy J. and Lucille A. Carver College of Medicine
Hypothesis
Can embryonic stem cell-derived hematopoietic progenitor cells induce transplantation tolerance without immunosuppressive agents?
Conclusion
ES-derived hematopoietic progenitor cells can engraft in allogenic recipients without immunosuppressive agents, protecting cardiac allografts from rejection.
Supporting Evidence
- HPCs poorly express MHC antigens, allowing long-term engraftment across MHC barriers.
- Chimeric animals accepted donor-type cardiac allografts without immunosuppression.
- Low level chimerism was maintained in the bone marrow over 100 days.
Takeaway
Scientists found a way to use special cells from embryos to help the body accept new organs without needing strong medicine to stop the body from fighting them.
Methodology
CD45+ hematopoietic progenitor cells were derived from HOXB4-transduced embryonic stem cells and transplanted into sublethally irradiated mice.
Potential Biases
Potential bias in the selection of animal models and the specific conditions under which experiments were conducted.
Limitations
The study does not address the long-term effects of using these cells in human patients.
Participant Demographics
Mice used included immunocompetent MRL and syngeneic 129SvJ strains.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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