Identifying a Gene Linked to Resistance in European Corn Borer
Author Information
Author(s): Khajuria Chitvan, Buschman Lawrent L., Chen Ming-Shun, Siegfried Blair D., Zhu Kun Yan
Primary Institution: Kansas State University
Hypothesis
Is the OnAPP gene involved in Cry1Ab toxicity and resistance in the European corn borer?
Conclusion
The OnAPP gene may play a significant role in binding the Cry1Ab toxin in the European corn borer's larval midgut, and mutations in this gene could be associated with resistance.
Supporting Evidence
- Expression analysis showed that most aminopeptidase-like genes were expressed predominantly in the larval midgut.
- RNA interference of the OnAPP gene resulted in reduced susceptibility to Cry1Ab.
- Mutations in the OnAPP gene were identified in Cry1Ab-resistant strains compared to susceptible strains.
Takeaway
Scientists studied a gene in corn pests to see if it helps them resist a specific toxin. They found changes in the gene that might help the pests survive.
Methodology
The study involved gene expression analysis, RNA interference assays, and bioassays to assess the role of the OnAPP gene in Cry1Ab toxicity.
Limitations
The study does not rule out the possibility that other factors contribute to resistance in the European corn borer.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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