Haemolysis Affects miRNA Levels in Plasma
Author Information
Author(s): Kirschner Michaela B., Kao Steven C., Edelman J. James, Armstrong Nicola J., Vallely Michael P., van Zandwijk Nico, Reid Glen
Primary Institution: Asbestos Diseases Research Institute (ADRI), University of Sydney
Hypothesis
Haemolysis may influence the quantification of microRNAs in plasma samples.
Conclusion
Haemolysis increases the levels of miR-16 and miR-451 in plasma, affecting their reliability as reference genes.
Supporting Evidence
- miR-16 levels were stable in non-haemolysed samples.
- Haemolysis led to an increase in miR-16 and miR-451 levels.
- RBCs were identified as the main source of miR-16 and miR-451 in plasma.
Takeaway
When blood samples are damaged, it can change the amount of certain tiny molecules called miRNAs in the blood, making it hard to use them for tests.
Methodology
The study measured miRNA levels in plasma samples from healthy individuals and patients with diseases, comparing haemolysed and non-haemolysed samples.
Potential Biases
Potential bias due to sample collection and processing methods.
Limitations
The study may not account for all variables affecting miRNA levels in plasma.
Participant Demographics
Included healthy volunteers and patients with malignant mesothelioma or coronary artery disease.
Statistical Information
P-Value
p<0.001
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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