Fetal membrane microbial flora at term: No association with chorioamnionitis
1996

Contamination in Fetal Membrane Cultures

Sample size: 17 publication Evidence: moderate

Author Information

Author(s): W51ner-Hanssen P, Morsing E, Higerstrand I, Hoist E, Ljung i, Herbst A

Primary Institution: Department of Obstetrics and Gynecology, University of Lund

Hypothesis

The high rate of bacterial growth in fetal membrane cultures is due to contamination from non-sterile gloves used by nurse-midwives.

Conclusion

The high rate of culture positivity reported previously is likely due to contamination from the gloves of the nurse-midwives.

Supporting Evidence

  • Culture specimens were obtained from 17 term women.
  • Nurse-midwives used the same gloves as during delivery.
  • 9 out of 17 cultures from nurse-midwives were positive.
  • Only 2 out of 17 cultures from the coauthor were positive.

Takeaway

When doctors took samples from pregnant women, they found that many of the bacteria were likely from dirty gloves, not from the women themselves.

Methodology

Controlled study comparing cultures obtained by nurse-midwives and a coauthor using sterile gloves.

Potential Biases

Potential bias from the handling of specimens by nurse-midwives.

Limitations

The study only included 17 cases and may not represent all term deliveries.

Participant Demographics

17 term women delivering during daytime.

Statistical Information

P-Value

0.02

Statistical Significance

p<0.05

Want to read the original?

Access the complete publication on the publisher's website

View Original Publication