Understanding the CRE1 Regulator in Trichoderma reesei
Author Information
Author(s): Portnoy Thomas, Margeot Antoine, Linke Rita, Atanasova Lea, Fekete Erzsébet, Sándor Erzsébet, Hartl Lukas, Karaffa Levente, Druzhinina Irina S, Seiboth Bernhard, Le Crom Stéphane, Kubicek Christian P
Primary Institution: IFP Energies nouvelles
Hypothesis
What are the gene targets regulated by the CRE1 carbon catabolite repressor in Trichoderma reesei?
Conclusion
The study reveals that CRE1 regulates a significant number of genes involved in carbon metabolism and that its function is influenced by the growth rate of the fungus.
Supporting Evidence
- 47.3% of the identified genes are repressed by CRE1.
- 29.0% of the genes are induced by CRE1.
- 17.2% of the genes are independent of CRE1 regulation.
- The study provides the first global insight into the molecular physiological response of a multicellular fungus to carbon catabolite regulation.
Takeaway
This study shows that a protein called CRE1 helps the fungus Trichoderma reesei decide which food to eat, depending on how fast it's growing.
Methodology
The researchers used genome-wide microarrays to analyze gene expression in wild-type and mutant strains of Trichoderma reesei under controlled growth conditions.
Limitations
The study primarily focuses on a limited number of genes and may not capture all regulatory interactions.
Statistical Information
P-Value
0.004
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
Want to read the original?
Access the complete publication on the publisher's website