Ribozyme-mediated inactivation of mutant K-ras oncogene in a colon cancer cell line
2000
Inactivation of Mutant K-ras in Colon Cancer Cells
publication
Evidence: moderate
Author Information
Author(s): Tokunaga T, Tsuchida T, Kijima H, Okamoto K, Oshika Y, Sawa N, Ohnishi Y, Yamazaki H, Miura S, Ueyama Y, Nakamura M
Primary Institution: Tokai University School of Medicine
Hypothesis
Can a hammerhead ribozyme effectively inactivate the mutant K-ras oncogene in colon cancer cells?
Conclusion
The study found that inactivating the mutant K-ras gene led to reduced growth and increased apoptosis in colon cancer cells.
Supporting Evidence
- KrasRz specifically cleaved the mutant K-ras mRNA but not wild-type mRNA in vitro.
- SW480/KrasRz showed decreased growth rate under tissue culture conditions.
- The xenotransplantability of SW480/KrasRz was significantly decreased in nude mice.
- Tumour volume of the xenografts XeSW480/KrasRz was significantly smaller than that of XeSW480/DisKrasRz.
- Gene expression of VEGF was suppressed in SW480/KrasRz.
- TSP1 gene expression was enhanced in SW480/KrasRz.
- SW480/KrasRz cells showed apoptosis-related features including nuclear condensation and DNA fragmentation.
Takeaway
Scientists used a special tool to turn off a bad gene in cancer cells, which helped stop the cells from growing and made them die.
Methodology
The study introduced a hammerhead ribozyme into colon cancer cells to evaluate its effects on growth and gene expression.
Statistical Information
P-Value
p<0.01
Statistical Significance
p<0.01
Digital Object Identifier (DOI)
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