Effects of DNA-targeted radiation on gene expression in human cells
Author Information
Author(s): Mykyta V. Sokolov, Ronald D. Neumann, Igor G. Panyutin
Primary Institution: Nuclear Medicine Department, Clinical Center, National Institutes of Health
Hypothesis
Does DNA-targeted ionizing radiation from 125I-IUdR induce specific changes in gene expression in primary human cells?
Conclusion
DNA-targeted ionizing radiation from 125I-IUdR leads to changes in the expression of a limited subset of genes in primary human cells, with a significant fraction being p53-dependent.
Supporting Evidence
- The study found that 335 genes were affected in gingival fibroblasts, while 49 and 27 genes were affected in fetal skin fibroblasts and keratinocytes, respectively.
- The majority of differentially expressed genes were involved in cell cycle regulation and DNA damage response.
- The expression changes were non-randomly distributed across chromosomes.
Takeaway
When human cells are exposed to a special type of radiation, only a few genes change their activity, and many of these changes are controlled by a protein called p53.
Methodology
The study used DNA microarrays to analyze gene expression changes in three primary human cell lines after exposure to 125I-IUdR.
Limitations
The study focused on only three cell lines, which may not represent all human cell types.
Participant Demographics
Three primary human cell lines: gingival fibroblasts, fetal skin fibroblasts, and neonatal foreskin epidermal keratinocytes.
Statistical Information
P-Value
<0.005
Statistical Significance
p<0.005
Digital Object Identifier (DOI)
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