Comparing Gene Expression in Corneal Neovascularization Models
Author Information
Author(s): Jia Changkai, Zhu Wei, Shengwei Xi, Haijie Li, Siyuan Wang, Yiqiang Wang
Primary Institution: Shandong Provincial Key Lab of Ophthalmology, Shandong Eye Institute, Shandong Academy of Medical Sciences, Qingdao, China
Hypothesis
This study compares the changes in genome-wide gene expression under suture placement and alkali burn conditions in murine corneal neovascularization models.
Conclusion
Sutures and alkali burns caused different types of damage to the cornea, but gene profiling revealed similar patterns of gene expression changes in both models.
Supporting Evidence
- Sutures caused localized damage while alkali burns resulted in total corneal opacity.
- 1,055 differentially expressed probes were identified in the suture model, while 861 were found in the alkali burn model.
- Both models showed overlapping gene expression changes, particularly in immune response and chemotaxis pathways.
Takeaway
The study looked at how two different ways to hurt the eye affect gene activity, and found that even though the damage looks different, the genes respond in similar ways.
Methodology
Corneal neovascularization was induced in Balb/c mice using either sutures or alkali burns, followed by microarray analysis of gene expression.
Limitations
The study focused on only two models of corneal neovascularization and may not represent all forms of the condition.
Participant Demographics
Balb/c mice, 6–8 weeks old.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Want to read the original?
Access the complete publication on the publisher's website