Negative auto-regulation increases the input dynamic-range of the arabinose system of Escherichia coli
2011

Negative Auto-Regulation Increases Input Dynamic Range in E. coli

publication Evidence: high

Author Information

Author(s): Madar Daniel, Dekel Erez, Bren Anat, Alon Uri

Primary Institution: The Weizmann Institute of Science

Hypothesis

Does negative auto-regulation preserve its function in a natural system embedded within many additional interactions?

Conclusion

Negative auto-regulation in the arabinose system of E. coli increases the range of arabinose signals that the system can respond to.

Supporting Evidence

  • Disrupting negative auto-regulation reduced the input dynamic range by about 10-fold.
  • The apparent Hill coefficient changed from about n = 1 with negative auto-regulation to about n = 2 when it was disrupted.
  • Negative auto-regulation allows the transcription factor concentration to be modulated by its own input signal.

Takeaway

This study shows that a special feedback mechanism in bacteria helps them respond better to different amounts of sugar.

Methodology

The study compared the wild type arabinose system to a variant with disrupted negative auto-regulation, measuring promoter activity in response to varying arabinose concentrations.

Digital Object Identifier (DOI)

10.1186/1752-0509-5-111

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