How Two Amino Acids Affect a Protein's Function at Low pH
Author Information
Author(s): Kakchingtabam Pushpa, Shahnaj Sharifun, Kumari Anju, Longjam Japani, Wungnaopam A. N., Herojit Khundrakpam, Laishram Rajendrakumar Singh, Fisher Aron B., Rahaman Hamidur
Primary Institution: Manipur University
Hypothesis
The formation of electrostatic interaction driven by the protonation of His79 and Asp42 because of lowering pH is necessary for the oligomerization process of Prdx6.
Conclusion
The study found that the amino acids Asp42 and His79 are crucial for the oligomerization and aiPLA2 activity of Prdx6 at low pH.
Supporting Evidence
- The wild type Prdx6 forms higher oligomers at low pH, which is essential for its aiPLA2 activity.
- Mutations at Asp42 and His79 significantly reduce aiPLA2 activity at low pH.
- The study utilized dynamic light scattering and size exclusion chromatography to analyze protein oligomerization.
Takeaway
This study shows that two specific parts of a protein help it change shape and work better when the environment is more acidic.
Methodology
The study used site-directed mutagenesis to create mutants of Prdx6 and analyzed their activity and oligomeric status at different pH levels.
Digital Object Identifier (DOI)
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