Cellulose-Binding Protein in Phytophthora
Author Information
Author(s): Richard W. Jones, Manuel Ospina-Giraldo
Primary Institution: United States Department of Agriculture-Agricultural Research Service
Hypothesis
Does Phytophthora infestans contain cellulose-binding domains associated with its cell wall proteins?
Conclusion
The study found that Phytophthora infestans has unique cellulose-binding proteins that are not associated with catalytic domains and do not elicit a plant hypersensitive response.
Supporting Evidence
- Only five genes encoding cellulose-binding domains were found in Phytophthora infestans.
- None of the identified proteins were associated with catalytic domains.
- Immunolocalization showed that one of the proteins, CBD1, is embedded in the hyphal cell wall.
Takeaway
The researchers looked for proteins in a plant pathogen that help it attach to plant cells, but they found that these proteins don't trigger a plant's defense system.
Methodology
A genome-wide survey for carbohydrate binding module family 1 (CBM1) was performed, followed by immunolocalization and expression tests in plants.
Limitations
The study did not identify any elicitor activity from the cellulose-binding protein CBD1, which was contrary to initial expectations.
Digital Object Identifier (DOI)
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