Angiogenically active vascular endothelial growth factor is over-expressed in malignant human and rat prostate carcinoma cells
2000
VEGF Over-Expression in Prostate Cancer Cells
Sample size: 7
publication
Evidence: moderate
Author Information
Author(s): Chen H J, Treweeke A T, Ke Y Q, West D C, Toh C H
Primary Institution: University of Liverpool
Hypothesis
Does vascular endothelial growth factor (VEGF) facilitate metastasis in prostate cancer by stimulating angiogenesis?
Conclusion
Malignant prostate cancer cells over-express VEGF, which is angiogenically active and may facilitate metastasis.
Supporting Evidence
- The level of VEGF mRNA in malignant cell lines was 3.5- to 10-fold higher than in benign cell lines.
- Metastatic variants expressed 2.5 to 5 times more VEGF mRNA than their parental benign cells.
- Malignant cells produced more VEGF protein than benign cells.
- CAM assay showed VEGF-dependent angiogenic activity in malignant cells.
Takeaway
This study found that cancer cells make a lot more of a substance called VEGF, which helps tumors grow and spread.
Methodology
The study used Northern and slot blotting, reverse transcription polymerase chain reaction, nucleotide sequence analysis, enzyme-linked immunosorbent assay, and the chick chorioallantoic membrane assay.
Digital Object Identifier (DOI)
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