Toll-Like Receptor 4 Is Involved in Inflammatory and Joint Destructive Pathways in Collagen-Induced Arthritis in DBA1J Mice
2011

TLR4's Role in Arthritis in Mice

Sample size: 34 publication 10 minutes Evidence: high

Author Information

Author(s): Matthias Pierer, Ulf Wagner, Manuela Rossol, Saleh Ibrahim

Primary Institution: University of Leipzig

Hypothesis

Does TLR4 deficiency affect the severity and incidence of collagen-induced arthritis in DBA1J mice?

Conclusion

TLR4 deficiency significantly reduces the incidence and severity of collagen-induced arthritis in mice.

Supporting Evidence

  • The incidence of arthritis was 59% in TLR4 deficient mice compared to 100% in wild type mice.
  • Mean maximum arthritis score was 2.54 in TLR4 deficient mice versus 6.25 in wild type.
  • TLR4 deficient mice showed virtually no cartilage destruction.
  • Lower concentrations of anti-CCP antibodies and IL-17 were found in TLR4 deficient mice.

Takeaway

Mice without TLR4 get less arthritis and have less joint damage when they are given a substance that usually causes arthritis.

Methodology

The study involved generating TLR4 deficient DBA1J mice and comparing their response to collagen-induced arthritis with wild type mice over a 90-day period.

Limitations

The study was conducted in mice, which may not fully replicate human rheumatoid arthritis.

Participant Demographics

DBA1J mice, both TLR4 deficient and wild type.

Statistical Information

P-Value

p<0.05

Statistical Significance

p<0.05

Digital Object Identifier (DOI)

10.1371/journal.pone.0023539

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