Detection of an oestrogen receptor-like protein in human meningiomas by band shift assay using a synthetic oestrogen responsive element (ERE)
1993

Estrogen Receptor-Like Protein in Human Meningiomas

Sample size: 15 publication Evidence: moderate

Author Information

Author(s): S.G.A. Koehorst, H.M. Jacobs, J.H.H. Thijssen, M.A. Blankenstein

Primary Institution: Academic Hospital Utrecht

Hypothesis

If the ER or ER-like protein is responsible for the induction of PR synthesis in meningiomas, then at least a protein should be present that can bind to the oestrogen responsive element (ERE) of the PR-gene.

Conclusion

The study found evidence for the existence of a protein that binds to the ERE in meningiomas, suggesting a potential role in the development and growth of these tumors.

Supporting Evidence

  • All but one of the meningiomas tested were positive for ER-ERE binding.
  • The presence of a protein that binds to the ERE suggests a role in PR synthesis.
  • Normal meninges were negative for ER-ERE binding, indicating a potential difference from tumor tissue.

Takeaway

The researchers looked for a protein in brain tumors called meningiomas that might help them understand how these tumors grow, and they found a protein that can stick to a special part of DNA.

Methodology

The study used a band shift assay to investigate the binding of meningioma cytosol components to a synthetic ERE.

Limitations

The study could not draw conclusions about the tumor specificity of the ER-ERE binding due to the lack of intact mRNA from normal meninges.

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