Impact of Canid Alphaherpesvirus 1 on Canine Stem Cells
Author Information
Author(s): Prišlin Šimac Marina, Naletilić Šimun, Kostanić Vjekoslava, Kunić Valentina, Zorec Tomaž Mark, Poljak Mario, Vlaj Doroteja, Kogoj Rok, Turk Nenad, Brnić Dragan
Primary Institution: Croatian Veterinary Institute, Zagreb, Croatia
Hypothesis
This research aimed to assess the impact of canid alphaherpesvirus 1 (CHV) on the functional attributes of canine adipose-derived mesenchymal stem cells (cAD-MSCs).
Conclusion
The study highlights the need for routine viral screening prior to the therapeutic use of cAD-MSCs due to the significant effects of CHV infection on their gene expression and secretome profile.
Supporting Evidence
- cAD-MSCs showed susceptibility to infection with wild-type CHV.
- Notable modifications in gene expression and secretome profile were observed post-infection.
- Gene expression changes indicated a shift towards an adipogenic phenotype.
Takeaway
This study found that a virus can change how dog stem cells work, which is important to know before using these cells for treatments.
Methodology
The study involved harvesting abdominal adipose tissue from 12 healthy dogs to isolate cAD-MSCs, which were then infected with a wild-type CHV strain and analyzed for gene expression and secretome changes.
Limitations
The study primarily focused on in vitro conditions, which may not fully replicate in vivo responses.
Participant Demographics
12 healthy dogs, 11 females and 1 male, aged between 6 to 60 months.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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