Effects of Hypoxia and Cobalt on Yeast Metabolism
Author Information
Author(s): Gleason Julie E., Corrigan David J., Cox James E., Reddi Amit R., McGinnis Lauren A., Culotta Valeria C.
Primary Institution: Johns Hopkins University Bloomberg School of Public Health
Hypothesis
How do hypoxia and cobalt exposure affect metabolic pathways in yeast?
Conclusion
Cobalt specifically targets certain iron proteins, while hypoxia affects amino acid regulation in yeast.
Supporting Evidence
- Cobalt treatment leads to a significant decrease in ergosterol levels in yeast.
- Hypoxia increases levels of certain amino acids while decreasing leucine and methionine.
- Cobalt specifically inhibits diiron-oxo enzymes involved in sterol synthesis.
- Hypoxia affects multiple enzymatic steps requiring oxygen and heme.
- Metabolite profiling reveals distinct impacts of cobalt and hypoxia on yeast metabolism.
Takeaway
This study shows that cobalt and low oxygen change how yeast cells use nutrients, but they do it in different ways.
Methodology
The study used metabolite profiling and gas chromatography/mass spectrometry to analyze yeast cells under different conditions.
Limitations
The study may not account for all metabolic pathways affected by hypoxia and cobalt.
Statistical Information
P-Value
0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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