Identification, cloning and characterization of a novel 47 kDa murine PKA C subunit homologous to human and bovine Cβ2
2006
Discovery of a New Protein in Mice Related to Human and Bovine PKA C Subunits
publication
Evidence: moderate
Author Information
Author(s): Ane Funderud, Heidi H. Henanger, Tilahun T. Hafte, Paul S. Amieux, Sigurd Ørstavik, Bjørn S. Skålhegg
Primary Institution: University of Oslo
Hypothesis
The study investigates the expression and characterization of a novel 47 kDa murine PKA C subunit that is homologous to human and bovine Cβ2.
Conclusion
Murine lymphoid tissues express a protein that is a homologue of human and bovine Cβ2.
Supporting Evidence
- The novel murine Cβ2 was shown to be an active protein kinase.
- Immunoreactivity confirmed the presence of the 47 kDa protein in lymphoid tissues.
- Sequence comparisons indicated that the murine Cβ2 is homologous to human Cβ2.
Takeaway
Scientists found a new protein in mice that is similar to a protein found in humans and cows, which helps cells respond to signals.
Methodology
The study involved cloning, expression, and characterization of a novel 47 kDa protein from murine tissues, along with immunoblotting and kinase activity assays.
Digital Object Identifier (DOI)
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