Comparative Analysis of Transcriptomic and Proteomic Profiles in Porcine Tissues
Author Information
Author(s): Hornshøj Henrik, Bendixen Emøke, Conley Lene N, Andersen Pernille K, Hedegaard Jakob, Panitz Frank, Bendixen Christian
Primary Institution: Aarhus University
Hypothesis
How do transcript and protein levels correlate across different porcine tissues?
Conclusion
The study found that while cDNA microarray and 454-sequencing technologies show high reproducibility within the same tissue, their agreement across technologies is modest, yet the regulation of transcript and protein levels is positively correlated.
Supporting Evidence
- 2,541 unique transcripts were detectable by both microarray and 454-sequencing.
- High reproducibility was observed within cDNA microarray (0.98) and 454-sequencing (0.85).
- 198 out of 306 differentially expressed genes identified by 454-sequencing were also found by microarray.
Takeaway
Scientists looked at how genes and proteins behave in pig heart and muscle tissues, finding that when one is high, the other usually is too.
Methodology
The study used cDNA microarray, 454-sequencing, and iTRAQ-based proteomics to analyze gene and protein expression in porcine heart and skeletal muscle tissues.
Potential Biases
Potential biases may arise from the differences in sensitivity and specificity of the technologies used for expression profiling.
Limitations
The study's findings may be limited by the specific tissues analyzed and the technologies used, which may not capture all aspects of gene and protein expression.
Participant Demographics
Tissue samples were taken from five healthy Hampshire gilts aged four to six months.
Statistical Information
P-Value
0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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