Reduced ALP Levels and Resistance to Cry Toxins
Author Information
Author(s): Jurat-Fuentes Juan Luis, Karumbaiah Lohitash, Jakka Siva Rama Krishna, Ning Changming, Liu Chenxi, Wu Kongming, Jackson Jerreme, Gould Fred, Blanco Carlos, Portilla Maribel, Perera Omaththage, Adang Michael
Primary Institution: University of Tennessee
Hypothesis
The study aims to identify a biomarker for resistance to Cry toxins in lepidopteran pests.
Conclusion
Reduced levels of membrane-bound alkaline phosphatase (mALP) are common in Cry-resistant strains of certain lepidopteran species.
Supporting Evidence
- Reduced mALP expression was detected in Cry-resistant H. virescens larvae compared to susceptible ones.
- Similar reductions in ALP activity were observed in Cry1Ac-resistant H. armigera and Cry1Fa-resistant S. frugiperda.
- Statistical analysis showed significant differences in ALP activity between resistant and susceptible strains.
Takeaway
Some bugs that are resistant to certain pesticides have lower levels of a specific protein, which could help scientists find out if other bugs are resistant too.
Methodology
The study used differential proteomics (2D-DIGE) and quantitative real-time PCR (qRT-PCR) to analyze protein and transcript levels in resistant and susceptible insect strains.
Limitations
The study may not account for all resistance mechanisms and the correlation between ALP activity and resistance is not fully established.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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