Characterization of hARD2, a human protein N-α-acetyltransferase
Author Information
Author(s): Thomas Arnesen, Matthew J Betts, Frédéric Pendino, David A Liberles, Dave Anderson, Jaime Caro, Xianguo Kong, Jan E Varhaug, Johan R Lillehaug
Primary Institution: University of Bergen
Hypothesis
The study investigates the characterization and function of the human protein hARD2, a duplicate of hARD1.
Conclusion
hARD2 is a human protein N-α-acetyltransferase that may complement the functions of hARD1, contributing to protein N-α-acetylation in human cells.
Supporting Evidence
- hARD2 has 81% sequence identity to hARD1.
- hARD2 mRNA and protein are expressed in several human cell lines.
- hARD2 potentially interacts with NATH, suggesting a role in protein N-α-acetylation.
- hARD2 protein levels remain stable during differentiation in NB4 cells.
Takeaway
Scientists found a new protein in humans called hARD2 that helps with a process called acetylation, which is important for how cells work.
Methodology
The study involved cloning and expression analysis of hARD2, immunoprecipitation experiments, and N-acetyltransferase activity assays.
Limitations
The study does not explore the full range of functions of hARD2 or its potential substrates in detail.
Digital Object Identifier (DOI)
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