Capsule Structure and Biogenesis in Vibrio cholerae NRT36S
Author Information
Author(s): Chen Yuansha, Bystricky Peter, Adeyeye Jacob, Panigrahi Pinaki, Ali Afsar, Johnson Judith A, Bush CA, Morris JG Jr, Stine OC
Primary Institution: University of Maryland, Baltimore
Hypothesis
The study aims to elucidate the capsule polysaccharide structure and biogenesis in non-O1 Vibrio cholerae NRT36S.
Conclusion
The co-location of CPS and LPS biosynthesis genes suggests a mechanism for the simultaneous emergence of new O and K antigens in a single strain, aiding V. cholerae in evading host immune detection.
Supporting Evidence
- The capsule structure was determined to be a complex structure with four residues in the repeating subunit.
- The gene cluster for capsule biogenesis was identified and found to share the same genetic locus as the O-antigen of LPS.
- This is the first complete elucidation of the CPS structure for a non-O1 Vibrio cholerae strain.
Takeaway
This study found that the capsule of Vibrio cholerae NRT36S is made up of different sugar units, and the genes that help make this capsule are located near the genes for another important structure, the LPS.
Methodology
The study used high-resolution NMR spectroscopy, transposon mutagenesis, and whole genome sequencing to analyze the capsule structure and identify the gene cluster responsible for its biogenesis.
Digital Object Identifier (DOI)
Want to read the original?
Access the complete publication on the publisher's website