SDS-PAGE Method for Screening Kidney Stone Disease
Author Information
Author(s): Wai-Hoe Lau, Wing-Seng Leong, Ismail Zhari, Lay-Harn Gam
Primary Institution: School of Pharmaceutical Sciences, Universiti Sains Malaysia
Hypothesis
Can Tamm Horsfall glycoprotein (THP) be reliably quantified using an SDS-PAGE-based method to screen for kidney stone disease?
Conclusion
The developed SDS-PAGE method can effectively distinguish between healthy individuals and kidney stone patients based on THP concentration.
Supporting Evidence
- The assay showed a sensitivity of 92.3% and specificity of 83.3%.
- THP concentration was significantly lower in stone patients compared to healthy subjects.
- The method was validated for reproducibility and repeatability with CVs of 4.8% and 2.6%, respectively.
- A distinct cut-off of 5.6 μg/mL THP concentration was established to differentiate between groups.
- Urinary THP was effectively recovered using ammonium sulphate precipitation.
Takeaway
Researchers created a simple test to check for kidney stones by measuring a special protein in urine. This test can help doctors tell if someone has kidney stones.
Methodology
Urinary proteins were extracted using ammonium sulphate precipitation and quantified using SDS-PAGE.
Limitations
The specificity of the method may be increased if used in conjunction with clinical symptoms.
Participant Demographics
117 healthy subjects (41 male, 76 female) aged 20-86 years and 58 stone patients (34 male, 24 female) aged 28-74 years.
Statistical Information
P-Value
0.0001
Confidence Interval
29.34–45.98 μg/mL for healthy males; 51.86–72.69 μg/mL for healthy females.
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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