A Large SV40 Particle Penetrates the ER Membrane
2011
How SV40 Virus Penetrates the Endoplasmic Reticulum to Infect Cells
publication
Evidence: high
Author Information
Author(s): Inoue Takamasa, Tsai Billy, Galloway Denise
Primary Institution: University of Michigan Medical School
Hypothesis
What is the mechanism by which the non-enveloped SV40 penetrates the endoplasmic reticulum membrane to access the cytosol?
Conclusion
SV40 penetrates the ER membrane as a large and intact particle, undergoing disassembly in the cytosol.
Supporting Evidence
- SV40 is released into the ER lumen and undergoes disulfide bond disruption to reach the cytosol.
- A large and intact viral particle penetrates the ER membrane despite conformational changes.
- VP3 and the viral genome are involved in regulating SV40's transport to the cytosol.
- The proteasome plays a critical role in the release of SV40 into the cytosol.
Takeaway
The SV40 virus can enter cells by passing through the endoplasmic reticulum, and it does this while still in one piece before breaking apart inside the cell.
Methodology
A cell-based assay was used to study the transport of SV40 from the endoplasmic reticulum to the cytosol.
Digital Object Identifier (DOI)
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