Corto and Cyclin G Interaction in Drosophila
Author Information
Author(s): Salvaing Juliette, Nagel Anja C., Mouchel-Vielh Emmanuèle, Bloyer Sébastien, Maier Dieter, Preiss Anette, Peronnet Frédérique
Primary Institution: Laboratoire de Biologie du Développement, UMR 7622, Centre National de la Recherche Scientifique (CNRS), Université Pierre et Marie Curie-Paris 6, Paris, France
Hypothesis
How does Cyclin G interact with the ETP Corto in Drosophila and what is its role during development?
Conclusion
CycG is essential for development in Drosophila and interacts with Corto to regulate gene expression.
Supporting Evidence
- Cyclin G was identified as a partner of Corto through a two-hybrid screen.
- Inactivation of Cyclin G by RNA interference led to developmental lethality in Drosophila.
- Corto and Cyclin G co-localize at multiple sites on polytene chromosomes.
- Both proteins bind to the regulatory element iab-7 PRE and the promoter of the Abd-B gene.
Takeaway
This study found that a protein called Cyclin G works with another protein called Corto to help control how genes are turned on and off during the development of fruit flies.
Methodology
The researchers used a two-hybrid screen and co-immunoprecipitation to study the interaction between Corto and Cyclin G.
Digital Object Identifier (DOI)
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