Spherezymes: A New Enzyme Technology
Author Information
Author(s): Brady Dean, Jordaan Justin, Simpson Clinton, Chetty Avashnee, Arumugam Cherise, Moolman Francis S
Primary Institution: CSIR Biosciences
Hypothesis
Can enzymes be effectively self-immobilised in an emulsion while retaining their activity?
Conclusion
The study successfully demonstrated that self-immobilised spherical enzyme particles, named spherezymes, can be created and retain activity for recycling in both aqueous and organic media.
Supporting Evidence
- The immobilised enzyme particles showed higher activity for small ester molecules compared to free enzymes.
- Particle size could be controlled by adjusting surfactant and protein concentration.
- The method allows for the recycling of enzymes without loss of activity.
- Co-immobilisation with other proteins was successfully demonstrated.
Takeaway
Scientists created tiny balls of enzymes that can be reused in different liquids, making them work better and last longer.
Methodology
Enzymes were self-immobilised in a water-in-oil emulsion using cross-linking agents, and their activity was tested in various conditions.
Limitations
The study may not address all potential enzyme types or conditions for industrial applications.
Digital Object Identifier (DOI)
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