KIF14 and E2F3 mRNA Expression in Retinoblastoma
Author Information
Author(s): Madhavan Jagadeesan, Mitra Moutushy, Mallikarjuna Kandalam, Pranav Oberoi, Srinivasan Ramalingam, Nagpal Amit, Venkatesan Perumal, Kumaramanickavel Govindasamy
Primary Institution: Sankara Nethralaya, Chennai, India
Hypothesis
The study aims to quantify mRNA expression of KIF14 and E2F3 in retinoblastoma tumors and associate it with disease phenotype.
Conclusion
The study confirms significant mRNA overexpression of KIF14 and E2F3 in retinoblastoma tumors, with decreased expression in chemotherapy-treated cases.
Supporting Evidence
- KIF14 and E2F3 mRNA were significantly overexpressed in retinoblastoma tumors compared to control retinas.
- E2F3 showed significant overexpression compared to RB cell lines.
- Chemotherapy-treated tumors had significantly decreased expression of KIF14 and E2F3.
Takeaway
The researchers looked at two genes in eye tumors and found they were more active in sick eyes than in healthy ones, especially in older kids.
Methodology
mRNA expression was quantified using real-time PCR in tumor samples and control retinas, with immunohistochemistry to confirm protein expression.
Limitations
The study's findings on chemotherapy effects need further validation in a larger cohort of treated cases.
Participant Demographics
Patients with retinoblastoma aged between 1 to 84 months.
Statistical Information
P-Value
p<0.0001
Statistical Significance
p<0.0001
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