Control of Gene Expression by RORα in HepG2 Cells
Author Information
Author(s): Chauvet Caroline, Vanhoutteghem Amandine, Duhem Christian, Saint-Auret Gaëlle, Bois-Joyeux Brigitte, Djian Philippe, Staels Bart, Danan Jean-Louis
Primary Institution: Université Paris Descartes, Paris, France
Hypothesis
To better understand the role of RORα in liver cells, we aimed to display genes controlled by RORα in HepG2 human hepatoma cells.
Conclusion
The study identified several novel genes regulated by RORα in HepG2 cells, suggesting its role in glucose metabolism and carcinogenesis.
Supporting Evidence
- RORα was found to regulate genes involved in lipid metabolism, inflammation, and circadian rhythm.
- SPARC was identified as a new target gene of RORα.
- Chromatin immunoprecipitation confirmed RORα binding to the regulatory regions of target genes.
- Micro-array analysis revealed significant changes in gene expression due to RORα over-expression.
Takeaway
Researchers found that a protein called RORα helps control important genes in liver cells, which could affect how the body processes sugar and fights cancer.
Methodology
The study used micro-arrays and qRT-PCR to analyze gene expression in HepG2 cells stably over-expressing RORα.
Statistical Information
P-Value
p<0.01
Statistical Significance
p<0.01
Digital Object Identifier (DOI)
Want to read the original?
Access the complete publication on the publisher's website